Phospho tbk1 antibody

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August undefined, 2024

WebDec 9, 2013 · TBK1 is phosphorylated in its activation loop, leading to IRF3/7 activation and Type I interferon (IFN) production. IPS-1 and STING localize to the mitochondria and endoplasmic reticulum,... WebHere we show that STING stimulates IRF3 phosphorylation by the kinase TBK1 in an in vitro reconstitution system. Using this system, we identified a carboxyl terminal region of STING that is both necessary and sufficient to activate TBK1 and stimulate IRF3 phosphorylation.

TBK1 [p Ser172] Antibody (EPR2867(2)) - Novus Biologicals

WebThe Phospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb Antibody has been validated for the following applications: Precipitation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blot. Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb detects endogenous levels of TBK1 only when phosphorylated … WebTBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct … cyclopropane functional group

Western Blot Detection of Phosphorylation Events - Bio-Rad

WebApr 10, 2024 · Importance of Validating Antibodies and Small Compound Inhibitors Using Genetic Knockout Studies-T Cell Receptor-Induced CYLD Phosphorylation by IKKε/TBK1 as a Case Study Importance of Validating Antibodies and Small Compound Inhibitors Using Genetic Knockout Studies-T Cell Receptor-Induced CYLD Phosphorylation by IKKε/TBK1 … WebRecently, a new IKK family member, TBK1 [ (TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner. Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. WebWestern blot - Phospho-TBK1/NAK-S172 Rabbit mAb (AP1026) Western blot analysis of extracts of various cell lines, using Phospho-TBK1/NAK-S172 Rabbit mAb (AP1026) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. cyclopropane hexane

(PDF) Cell Type-Specific Subcellular Localization of Phospho-TBK1 …

Phospho-TBK1 (Ser172) antibody (82383-1-RR) Proteintech

WebThis phospho-TBK1 antibody is validated for use in WB for the detection of the phospho-TBK1 protein. Quality Evaluated by Western Blot in EGF treated and untreated HEK293 cell … WebJan 30, 2024 · TBK1 Antibody (A-6) is a mouse monoclonal IgG 1 κ TBK1 antibody, cited in 7 publications, provided at 200 µg/ml. raised against amino acids 355-729 mapping at the C … cyclopropane induction picsWebDec 10, 2015 · Phospho-TBK1 and total TBK1 were also present in additional fractions; correlating with the observation that phospho-TBK1 also is associated with spindle … cyclopropane isopropenyl

"WebImmunogen: Phosphorylated Human TBK1 Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_647214 Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% … " - Phospho tbk1 antibody

Phospho tbk1 antibody

Importance of Validating Antibodies and Small Compound ... - PubMed

WebProduct Details Antibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: T2K, NAK Reactivity: Human (QC Testing) Isotype: Mouse IgG1, κ Immunogen: Human TBK1 C terminus Recombinant Protein Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_1645537 WebWestern blot - Phospho-TBK1/NAK-S172 Rabbit pAb (AP0847) Western blot analysis of HeLa, using Phospho-TBK1/NAK-S172 antibody (AP0847) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit …

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WebPhospho-TBK1 (Ser172) Antibody (PA5-105919) in IHC (P) Immunohistochemistry analysis of paraffin-embedded Phospho-TBK1 (Ser172) in mouse kidney tissue sections. … WebTBK1 Background NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor−associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity.

WebWhile TBK1 is known to phosphorylate cargo receptors to promote ubiquitin binding, it is unknown whether TBK1 phosphorylates other proteins to promote mitophagy. Using global quantitative proteomics, we identified S72 in RAB7A, a RAB previously linked with mitophagy, as a dynamic target of TBK1 upon mitochondrial depolarization. WebDec 1, 2024 · AMPK directly phosphorylates TBK1 at S511 to prime antiviral sensing • AMPK-TBK1 couples glucose deficiency sensing and innate immune surveillance • Targeting the AMPK-TBK1 axis compromises innate antiviral immunity Summary Nutrient sensing and damage sensing are two fundamental processes in living organisms.

WebAnti-phospho-TBK1 (Ser172) Antibody 07-2192 Sigma-Aldrich Anti-phospho-TBK1 (Ser172) Antibody Download Zoom This phospho-TBK1 antibody is validated for use in WB for the … WebSerine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1, TBK1, NAK: Target/Specificity : This TBK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S172 of human TBK. Dilution : WB~~1:1000 DB~~1:500: Format

WebPhospho-TBK1 (Ser172) Antibody (MA5-35869) in WB Western Blot analysis of Phospho-TBK1 (Ser172) performed on various cell lines using a Phospho-TBK1 (Ser172) monoclonal antibody ( Product # MA5-35869) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000. Product Details

WebMonoclonal Antibody for studying TBK1 (Ser172) phosphate. Cited in 553 publications. Validated for WB, IP, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit Monoclonal Antibody (CST #5483) is ready to ship. cyclopropane melting pointWebPhospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb (PE Conjugate) recognizes endogenous levels of TBK1 only when phosphorylated at Ser172. This antibody may … cyclopropane isoproyl etherWebWestern blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml), up to 24h, using Phospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower). Show More cyclopropane hydrate 1:1 densityWebThe Phospho-TBK1 (Ser172) assay measures TBK1 when phosphorylated at Ser172. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-TBK1 (Ser172) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. cyclopropane molecular weightWebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved sensitivity … cyclopropane isomersWebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free production For … cyclopropane mycolic acid synthaseWebPhospho-TBK1/NAK-S172 Rabbit mAb (AP1026) Western blot analysis of extracts of various cell lines, using Phospho-TBK1/NAK-S172 Rabbit mAb (AP1026) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were … cyclopropane radical ring opening