Cryptic plasmid

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August undefined, 2024

WebApr 7, 2024 · A plasmid is a small, circular piece of DNA that is different than the chromosomal DNA, which is all the genetic material found in an organism’s chromosomes. It replicates independently of chromosomal …

Plasmid - Wikipedia

WebPolypeptides encoded by cryptic plasmids from Neisseria gonorrhoeae. Plasmid. 1985 Nov;14(3):209–216. [PubMed] [Google Scholar] Aalen RB, Gundersen WB. Molecular characterization and comparison of plasmid content in seven different strains of Neisseria gonorrhoeae. Acta Pathol Microbiol Immunol Scand B. 1987 Feb;95(1):13–21. WebFeb 20, 2024 · The cryptic plasmid is important for chlamydial colonization in the gastrointestinal tract. We used a combination of intragastric, intrajejunal, and intracolon … circle k fernandina beach

The complete nucleotide sequence of a small cryptic plasmid …

WebMay 21, 2009 · The plasmid from the new Swedish variant has a 377 bp deletion in the first predicted coding sequence, abolishing the site used for PCR detection, resulting in negative diagnosis. In addition, the variant plasmid has a 44 … Some plasmids, however, have no observable effect on the phenotype of the host cell or its benefit to the host cells cannot be determined, and these plasmids are called cryptic plasmids. Naturally occurring plasmids vary greatly in their physical properties. See more A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double … See more Plasmids may be classified in a number of ways. Plasmids can be broadly classified into conjugative plasmids and non-conjugative plasmids. Conjugative plasmids contain a set of transfer genes which promote sexual conjugation between different … See more The term episome was introduced by François Jacob and Élie Wollman in 1958 to refer to extra-chromosomal genetic material that may … See more Some plasmids or microbial hosts include an addiction system or postsegregational killing system (PSK), such as the hok/sok (host … See more The term plasmid was introduced in 1952 by the American molecular biologist Joshua Lederberg to refer to "any extrachromosomal hereditary determinant." The … See more In order for plasmids to replicate independently within a cell, they must possess a stretch of DNA that can act as an origin of replication. The self-replicating unit, in this case, the plasmid, is called a replicon. A typical bacterial replicon may consist of a number … See more Artificially constructed plasmids may be used as vectors in genetic engineering. These plasmids serve as important tools in genetics and biotechnology labs, where they are commonly … See more WebJun 1, 1988 · The plasmid was cloned at the BamHl site of pAT153 into Escherichia coli and the recombinant plasmid was designated pCTL1. A detailed restriction endonuclease map of pCTL1 was constructed. circle k feet

Cryptic plasmid pRK2 from - ScienceDirect

Co-evolution of genomes and plasmids within - BioMed Central

WebSep 1, 2007 · However, sequence homology with any phenotype marker gene (e.g., metal resistance) could not be detected suggesting this plasmid to be cryptic in nature. This article is the first to report the complete nucleotide sequence analysis of a plasmid from A. multivorum JCM8867 as well as from any Acidiphilium strain. In this study, we present … WebA 4.8 kb Pst I fragment of pRmeGR4a, responsible for the high stability in cis of this plasmid, has been isolated and several recombinant plasmids have been constructed showing different segregation rates in the strains used in this study. Their stabilities can be considerably improved by insertion of the stabilization mrs/par region of RK2. circle k farrow roadWebNational Center for Biotechnology Information diamond and ruby eternity ring

"WebApr 11, 2024 · A plasmid to which no phenotypic traits have been ascribed. From: cryptic plasmid in Oxford Dictionary of Biochemistry and Molecular Biology » Subjects: … " - Cryptic plasmid

Cryptic plasmid

WebApr 11, 2024 · Curing of this cryptic plasmid from a bacterial strain is a method to substantiate the relationship between a genetic trait and carriage of that specific trait in … WebFeb 10, 2024 · For genetic complementation and ectopic gene expression in this bacterium, scientists currently rely on only shuttle plasmids derived from the cryptic plasmid pWH1277 originally isolated from A. calcoaceticus . Replication of these plasmids in Escherichia coli was achieved by incorporating such origin of replication as the ColE1 . These ...

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WebApr 13, 2024 · Background Within the genus Escherichia, several monophyletic clades other than the traditionally defined species have been identified. Of these, cryptic clade I (C-I) appears to represent a subspecies of E. coli, but due to the difficulty in distinguishing it from E. coli sensu stricto, the population structure and virulence potential of C-I are unclear. … WebSep 1, 1989 · The plasmid, designated pC30i1, has a molecular size of 2140 bp and a GC content of 37%. The sequence contains one major open reading frame (ORF R) of 951 bp, encoding a basic polypeptide of 317 amino acids, and a molecular weight of 36,956.

WebJun 1, 2006 · A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture Microbiology Society Volume 152, Issue 6 Other Free A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture Catherine M. O'Connell 1, Kristy M. … WebThe cryptic plasmid, pWV01, exhibits broad host range but is unstable in many species. The ORF D, and inverted repeats IV, V and VI were deleted from its plasmid origin; terminators t0 and T1...

WebJan 8, 2024 · Plasmid:chromosome ratio Cohort 1 The tPCr varied from 1 to 677. The majority of samples (217; 57%) contained ≤ 18 plasmids per chromosome, comparable … Web质粒载体网zlzt.com专业提供pSEAP2-Control,产品编号为zl-011128,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质粒合成,质粒构建,质粒提取,质粒保藏!

WebMar 9, 2024 · A cryptic plasmid, pLU4, was isolated and sequenced from Lactobacillus reuteriLactobacillus species. A region encoding a cluster of conjugative transfer genes (trs or tra) in pLU4 showed high similarity and co-linearity with the trs region of plca36 and tra region of pLgLA39 from L. casei str. Zhang and L. gasseri, respectively.This is the first …

WebMar 1, 2024 · Here, we identified a small cryptic plasmid (designated pAhX22) in A. hydrophila strain X22. pAhX22 was used as backbone for the construction of a new shuttle vector, pAEsv-1, for Escherichia coli and A. hydrophila. The gfp gene was successfully stably expressed in A. hydrophila X2 using pAEsv-1. diamond and ruby necklace tiffanyWebMar 12, 2024 · Most gonococci contain the cryptic plasmid, and the absence of this plasmid has been reported to be associated with a novel chromosomal type IV secretion system found in N. gonorrhoeae [62].... circle k find a storeWebDual target detection of the 16S rRNA gene and a cryptic plasmid SIMPLE LABORATORY WORKFLOW Easily combinable with other PCR kits from the STI Panel in one workflow EASY-TO-USE CONCEPT Single tube Ready-to-Use Master Mix contains all components for PCR amplification No additional PCR reagents pipetting necessary diamond and ruby necklaceWebThe plasmids pAsa1, pAsa2, and pAsa3 possess only the genes required for their replication and mobilization. Given that no function has been assigned to them yet, these plasmids are called “cryptic.” pAsa1 and pAsa3 also have genes involved in their stability since they harbor a toxin-antitoxin (TA) system ( Boyd et al., 2003 ). diamond and rumealWebThese new observations suggest that transposons and particularly Tn 1721 are frequent and diversified in A. salmonicida subsp. salmonicida. Moreover, the discovery of pAsaXI and … circle k firearms mnWebA 7.5 kbp cryptic plasmid is found in almost all isolates of Chlamydia trachomatis. Real-time PCR assays, using TaqMan chemistry, were set up to quantify accurately both the … diamond and ruby pendant necklaceWebMar 18, 2016 · Plasmids are essential tools for gene transfer and manipulation in many kinds of microorganisms, but they remain mysterious in the Shewanella species. Herein, a novel cryptic plasmid pSXM33 was isolated from marine bacterium Shewanella xiamenensis BC01 (SXM) and followed by sequencing and characterization through … diamond and ruby wedding band